Nicotine Screening

Nicotine Urine Test Kit

Cotinine TestThe One-Step Rapid Nicotine Test is an easy-to-use instant urine test that detects cotinine in human urine. Cotinine is a metabolite left over after the body processes nicotine and is the preferred way to test for nicotine use.

These simple and convenient tests give you fast, qualitative results, meaning they will simply tell you whether or not cotinine has been detected in the body over the 200 ng/ml cut-off concentration.

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Features and Benefits:

  • Easy to use
  • Reliable results
  • Instant urinalysis

How to Use:

Positive: One pink-rose band appears in the control zone and no band appears in the test zone (for a total of one band). A positive result indicates the Cotinine level is 200 ng/ml or higher in the test urine sample.

Negative: One pink band appears in the control zone and one band appears in the test zone (for a total of two bands). A negative result indicates that the Cotinine level is below the detection sensitivity of 200 ng/ml.

IMPORTANT: Any line, regardless of how faint, appearing in the test area is a negative test result.

The test is invalid if there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone. (Control zone band must show up.) In this instance, re-testing of the specimen is recommended.

Most experts and healthcare professionals agree that nicotine is unquestionably the most addictive drug in use today. The principle source of nicotine is tobacco products. Tobacco smoking, vaping of liquids that contain nicotine or chewing of tobacco results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into 20 different metabolites excreted in urine.

Cotinine, a major metabolite of nicotine, accumulates in the body with regular smoking. It is reported that Cotinine is stable in body fluids and has a relatively long half-life of approximately 17 hours. Therefore the detection of Cotinine is less dependent on the time of sampling than that of nicotine and other metabolites.

Cotinine has been widely used as a bio-marker of tobacco exposure. Methods of analysis for Cotinine in biological fluids include gas chromatography, gas chromatography-mass spectrometry, HPLC, HPLC-mass spectrometry, EIA and RIA. These methods usually require special equipment and complicated operation procedures.

Product Insert (PDF)

Cotinine Test Product Insert

The One-Step Rapid Nicotine Test is a simple immunoassay test that is used for the qualitative detection of Cotinine in human urine
Brand: Confirm BioSciences