Nicotine Screening

Nicotine Screening

Cotinine Test


The One-Step Rapid Nicotine Test is a one step immunoassay that is used for the qualitative detection of Cotinine in human urine. It is based on the principle of highly specific immunochemical reactions of antigens and antibodies. It is a simple and convenient test for the rapid qualitative detection of Cotinine in human urine at 200 ng/ml cut-off concentration

Most experts and healthcare professionals agree that nicotine is unquestionably the most addictive drug in use today. The principle source of nicotine is tobacco products. Tobacco smoking or chewing results in the absorption of nicotine through the lung and buccal/nasal epithelium, after which nicotine is metabolized into 20 metabolites excreted in urine. Cotinine, a major metabolite of nicotine accumulates in the body with regular smoking. It is reported that Cotinine is stable in body fluids and has a relatively long half-life of approximately 17 hours. Therefore the detection of Cotinine is less dependent on the time of sampling than that of nicotine and other metabolites.

Cotinine has been widely used as a bio-marker of tobacco exposure. Methods of analysis for Cotinine in biological fluids include gas chromatography, gas chromatography-mass spectrometry, HPLC, HPLC-mass spectrometry, EIA and RIA. These methods usually require special equipment and complicated operation procedures

Features and Benefits:

  • Easily, Reliably, & Instantly Detect Tobacco Usage
  • Quick Analysis-on-site results in 5 minutes

How to Use:

Positive One (1) pink-rose band appears in the control zone and no band appears in the test zone. A positive result indicates the Cotinine level is 200 ng/ml or higher in the test urine sample.
Negative One band appears in the test zone and one band appears in the control zone. A negative result indicates that the Cotinine level is below the detection sensitivity of 200 ng/ml. IMPORTANT: any line, no matter how faint, appearing in the test area confirms a negative test.
Invalid If there are no distinct color bands visible in both the test zone and the control zone or if there is a visible band in the test zone but not in the control zone, then the test is invalid. In this instance, re-testing of the specimen is recommended.

Product Insert (PDF)

Cotinine Test Product Insert